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| Xie,LJ; Xie,XZ; Zhou,YW; Liang,C; Jiang,YY; Chen,Z. |
| ABSTRACT We investigated the effect of heat stress (HS) on the expression of the GABA receptor in the hypothalamic-pituitary-gonadal (HPG) axis of Wenchang chickens. Real-time quantitative RT-PCR (qRT-PCR) was used to quantify the GABA receptor mRNA levels along the HPG axis of chickens under HS (40±0.5 °C) for 1-6 weeks. Our results showed that the expression of GABAA and GABAB receptor at the mRNAs levels in the tissues of HPG axis exhibited fluctuation and variability. After HS, the mRNA level of GABAA receptor was significantly reduced in the hypothalamus of 1-week-old and in the pituitary of 3-week-old chickens, but significantly increased in the pituitary of 1-, 4-, and 5-week-old chickens. The GABAB receptor mRNA level significantly declined in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heat Stress; GABA receptor mRNA; HPG axis; Chickens. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200277 |
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| Xie,XZ; Liang,C; Li,MH; Chen,Z. |
| ABSTRACT Under routine feeding conditions, 1-day-old male chicks were randomly divided into control group (CK), heat-stress group (HS), and GABA + heat stress group (GABA+HS). The thymus contents of IL-1, IL-2, TGF-β1, IFN-γ, GH and HSP70 were determined by enzyme-linked immunosorbent assays. The results showed that: (1) IL-1 and TGF-β1 contents of HS group were significantly lower than those of the CK group (p<0.05), and those of the GABA+HS group were significantly higher relative to the HS group (p<0.05); (2) IL-2 and IFN-γ contents of the HS group were significantly higher than those of the CK group (p<0.05), and those of the GABA+HS group were significantly lower relative to the HS group (p<0.05);(3) the thymus GH content of all three... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heat stress; GABA; Cytokines; Thymus; Chicks. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000100143 |
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| Li,M; Wu,J; Chen,Z. |
| ABSTRACT One-day old chicks were randomly distributed into acute heat stress (AHS) or persistent heat stress (PHS) groups. Each group was further divided into control (CK), and three AHS ages (1, 2,or 3 weeks of age) experimental subgroups. The chicks in AHS subgroups were submitted to acute heat stress (40oC for two hours between 12:00 and 14:00 hours during the weekend) and the effects of heat stress on several daily behaviors were observed. At 8 days of age, the chicks of PHS subgroups were submitted to heat stress (40oC daily). The heat treatment ceased during the weekends and the effects on the behavior were observed three times daily for three consecutive days. The results showed that, compared with the CK group, the duration and frequency of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heat stress; Broiler behavior; Behavioral observations; Wenchang chicken. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400559 |
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| Liang,W; Lu,BB; Liang,C; Yu,ZQ; Xie,XZ; Chen,Z. |
| ABSTRACT This study conducted an in-depth investigation on the development of GABAergic neurons and their receptors in HPG axis-related target organs of Wenchang chicks under heat stress. One-day-old healthy Wenchang chicks were randomly divided into control (CK) and heat stress (HS) groups. Chicks in the HS group were placed in a 40±0.5°C climatic chamber for HS treatment from 13:00 to 15:00 daily. By immunohistochemistry and Western blotting, GABA and GABAA receptor (GABAAR) expression in the hypothalamus of the HS group was significantly higher (p<0.05), but GABAB receptor (GABABR) expression was significantly lower than that of the CK group (p<0.05). Expression of GABA and its two receptors in the pituitary tissues of the HS group was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Heat stress; GABAergic neurons; HPG axis; Wenchang chick. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200342 |
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